Richard Wigglesworth won his first match as interim head coach at Leicester as they beat Gloucester 28-8.
An Adam Hastings penalty was bettered by two from Freddie Burns to give Leicester the lead, before Albert Tuisue's try edged Gloucester ahead.
Guy Porter and an Anthony Watson double swung the lead definitively the Tigers' way in the second half, before Jonny May scored a late consolation.
The win meant Leicester also lifted the inaugural Slater Cup.
The win means Leicester overtake the Cherry and Whites in the Premiership table by two points and move up to fourth.
Gloucester head coach George Skivington made wholesale changes after coming in for criticism for resting the majority of his first-choice team in the Champions Cup thrashing by Leinster last week, with only Tuisue remaining in the starting XV.
It was the Fijian who made an early impact, winning a turnover inside two minutes and then crossing the try line, only to be held up.
Hastings put Gloucester's first points on the board, with the only downside for the visitors in a dominant opening 10 minutes being the loss of Wales winger Louis Rees-Zammit, who hobbled off with an ankle injury six weeks before the start of the Six Nations.
Burns retaliated with two penalties of his own to edge Leicester in front, but in a cagey half, Dan Kelly had a try scrubbed off for the Tigers.
Jack Van Poortvliet had darted through a gap in the Gloucester defence and offloaded to Kelly to run over, only for the officials to rule that Dan Coles held Tuisue's ankle in the build-up, allowing the initial space to open.
With the clock in red at the end of the half, Tuisue made Leicester pay and stretched over to score from a driving maul.
Porter instantly put Leicester back in front after the break, darting around the Gloucester line on the wing and then running between two defenders to cross the line.
It proved a hugely physical match with limited chances at either end before Watson capitalised as Leicester turned up the pressure, darting inside from close range to stretch the lead to 15 points.
The winger dived over in the corner for his second nine minutes later and while May ran in for his first try of the season, it was too little too late for Gloucester.
The first Slater Cup
Former Gloucester and Leicester lock Slater, 34, was diagnosed with motor neurone disease in June and was in the stand at Welford Road to watch the match with his family.
He presented Leicester captain Hanro Liebenberg with the trophy, which will be contested across each Premiership match between the two sides.
Five per cent of the price of each ticket went towards the 4ED Foundation, a campaign set up by Slater in the wake of his diagnosis, which aims to raise money towards his treatment and wider awareness of MND.
Leicester: Steward, Watson, Porter, Kelly, Potter, Burns, Van Poortvliet; Whitcombe, Clare, Cole, Wells, Chessum, Liebenberg (c), Refell, Cracknell.
Replacements: Oghre, Leatigaga, Heyes, Green, Jansen, Youngs, Atkinson, Ashton.
Gloucester: Carreras, Rees-Zammit, Harris, Atkinson, May, Hastings, Varney; Rapava-Ruskin, Socino, Gotovtsev, Clarke, Alemanno, Ackermann, Ludlow (c), Tuisue.
Replacements: McGuigan, Seville, Knight, Craig, Morgan, Chapman, Kveseladze, Evans.
Referee: Christophe Ridley