Wales scrum-half Kieran Hardy has been ruled out of the rest of the Six Nations after damaging a hamstring in the Triple Crown win against England.
Hardy scored a try before limping off at the Principality Stadium in the second half of the 40-24 victory.
Wales are confident Cardiff Blues' Tomos Williams will be fit again after suffering a hamstring injury in the opening victory against Ireland.
Wales hope fly-half Dan Biggar will be fit to face Italy on 13 March.
The Northampton half-back limped off against England and was replaced by Callum Sheedy who produced an impressive 13-point performance. Wales will find out next week if Biggar will be available.
At scrum-half, Wales should have three selection options in Gareth Davies, Tomos Williams and Lloyd Williams with nobody called up to replace Hardy.
His Scarlets' team-mate Davies came on as a replacement against England, while Lloyd Williams was called into the squad as cover for namesake Tomos.
Wales clinched the Triple Crown with victory over England and can win the Grand Slam by defeating Italy in Rome and France in Paris on 20 March.
Dragons back rower Aaron Wainwright and Ospreys centre Owen Watkin have been released by Wales to play for the regional sides in the Pro14 derby on Saturday evening.
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