British number eight Liam Broady has missed out a place in the main draw of Wimbledon after losing 3-6 0-6 6-2 6-4 6-3 to Frenchman Gregoire Barrere in the final round of qualifying.
He had been two sets up after 50 minutes against Barrere, ranked 170 places higher at 117 in the world.
Broady's best run at Wimbledon was reaching the second round in 2015.
In women's qualifying, Britain's Samantha Murray lost 4-6 6-2 3-6 to Spain's Paula Badosa.
Germany's 2013 Wimbledon finalist Sabine Lisicki also failed to make the main draw after she lost 0-6 6-4 6-4 to Lesley Kerkhove of the Netherlands.